mouse anti human integrin beta 3 antibody  (MedChemExpress)

Journal: Journal of Translational Medicine

Article Title: A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay

doi: 10.1186/1479-5876-2-4

Figure Lengend Snippet: Layered Expression Scanning (LES). (A) Ten disks (nine CAM specimens and one plain filter disk) were arrayed out on a glass slide. (B) A series of membranes (Multiblot membrane stack, 20/20 Gene Systems, Inc.) were generated and stained with polyclonal antibodies: rabbit anti-p21, rabbit anti-MetAP-2, mouse anti-human integrin alpha v beta 3, rabbit anti-Caspase-3. Images were scanned and analyzed on a Scan Array Express Microarray Scanner (Packard Biosciences). Images were corrected for total protein and loading correction. (C) LES/Fumagillin pathway. P21 staining was found to be greatest in CAM disks stimulated with bFGF and treated with systemic fumagillin. This was found to be highly significant when compared to stimulated CAM disks treated with systemic carrier solution ( P < 0.0001 by Student's T test). (D) MetAP-2 staining was strongest in CAM disks stimulated with bFGF and treated with systemic fumagillin ( P = 0.0042, Alternate Welch t test) and systemic LM609 ( P = 0.0253, Alternate Welch t test), compared to stimulated CAM disks treated with systemic carrier.

Article Snippet: LES used the following antibodies: rabbit anti-MetAP-2 (Zymed Laboratories Inc., 1:100 dilution, Cat#71–7200), rabbit anti-p21 (Zymed Laboratories Inc., 1:100 dilution, Cat#71–1000), mouse anti-human integrin alpha v beta 3 (Chemicon Intl., 1:500 dilution, Cat# MAB1976B), and rabbit anti-caspase-3 (Cell Signaling Technology, 1:500 dilution, Cat# 9661).

Techniques: Expressing, Generated, Staining, Microarray

Journal: Journal of Translational Medicine

Article Title: A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay

doi: 10.1186/1479-5876-2-4

Figure Lengend Snippet: LES/LM609 pathway. (A) Alpha v beta 3 staining was most intense in stimulated CAM disks. The difference in staining was found to be statistically significant between stimulated CAM disks and disks treated with fumagillin ( P = 0.01, Student's t test). (B) Cleaved caspase-3 staining was found to be greatest in stimulated disks treated with carrier solution and least in CAM disks treated with fumagillin ( P = 0.007, Student's t test) and LM609 ( P = 0.014, Student's t test).

Article Snippet: LES used the following antibodies: rabbit anti-MetAP-2 (Zymed Laboratories Inc., 1:100 dilution, Cat#71–7200), rabbit anti-p21 (Zymed Laboratories Inc., 1:100 dilution, Cat#71–1000), mouse anti-human integrin alpha v beta 3 (Chemicon Intl., 1:500 dilution, Cat# MAB1976B), and rabbit anti-caspase-3 (Cell Signaling Technology, 1:500 dilution, Cat# 9661).

Techniques: Staining

Journal: Journal of Translational Medicine

Article Title: A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay

doi: 10.1186/1479-5876-2-4

Figure Lengend Snippet: LES/ CC5079. (A) p21 was increased in CAM disks treated with systemic CC 5079 as compared to control ( P < 0.0001, Student's t test). (B) MetAP-2 trended towards greater expression in CAM disks after systemic CC 5079 injection, but this did not reach statistical significance compared to control ( P = 0.0787, Student's t test). (C) CC5079 increased the expression of cleaved caspase-3 ( P = 0.0184, Alternate Welch t test) while (D) inhibiting the expression of alpha v beta 3 ( P = 0.0266, Alternate Welch t test).

Article Snippet: LES used the following antibodies: rabbit anti-MetAP-2 (Zymed Laboratories Inc., 1:100 dilution, Cat#71–7200), rabbit anti-p21 (Zymed Laboratories Inc., 1:100 dilution, Cat#71–1000), mouse anti-human integrin alpha v beta 3 (Chemicon Intl., 1:500 dilution, Cat# MAB1976B), and rabbit anti-caspase-3 (Cell Signaling Technology, 1:500 dilution, Cat# 9661).

Techniques: Expressing, Injection

Journal: Journal of Hematology & Oncology

Article Title: Alpha-enolase (ENO1) controls alpha v/beta 3 integrin expression and regulates pancreatic cancer adhesion, invasion, and metastasis

doi: 10.1186/s13045-016-0385-8

Figure Lengend Snippet: ENO1-silencing modulates ECM-receptor expression. a Using quantitative PCR, mRNA coding for different proteins was investigated in shENO1 CFPAC-1 cells. Values are expressed as relative expression compared to control cells. b Western blot analysis was carried out to investigate alpha 5, beta 1, alpha v, beta 3, and uPAR expression on total lysates of shCTRL and shENO1 cells. c To determine surface expression of integrins, shENO1, or shCTRL cells were incubated with primary antibodies ( gray peak ) against beta 1, alpha v/beta 3, alpha IIb/beta 3, and or isotype-matched control antibody ( empty peak ) and analyzed by flow cytometry. d The adhesion ability of shENO1 and shCTRL cells on vitronectin was evaluated in the presence of anti-beta 3 Ab. Adherent cells were fixed with 2% glutaraldehyde in PBS and visualized by staining with crystal violet. For quantitative analysis, cells were treated with 10% acetic acid and elutes were read with a microplate reader at a wavelength of 570 nm. Results are expressed as OD, optical density units. A representative of three independent experiments is shown. Data are mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 relative to control cells

Article Snippet: A total of 1 × 10 5 cells were incubated with primary antibody: mouse IgG1 anti-human CD51/61 (alpha v/beta 3 integrin, BD, Milan, Italy), mouse IgG2a anti-human CD41/61 (alpha IIb/beta 3 integrin, BioLegend by Campoverde, Milan, Italy), mouse IgG1 anti-human beta1 integrin (Santa Cruz Biotechnology) or an isotype-matched negative control IgG1 or IgG2a antibody (Ab) accordingly (Dako, Milan, Italy), all at doses of 10 μg/ml for 30 min at 4 °C.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Incubation, Flow Cytometry, Staining